Browsing by Author "Rajput, D"

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    Current scenario and challenges in recycling of human urine generated at source in rail coaches as resource
    (2023-07) Dubey, KK; Rajput, D; Baldia, A; Kumar, A; Kumar, V; Yadav, A
    The current scenario of human urine being directly discharged into the environment without recycling, despite being an economical source of fertilizer. Train coaches are the major source of large-scale urine waste generation. Adopting a cir cular economy creates significant synergies toward usages of water generated after nutrient recovery from urine. Some advanced decentralized treatment systems, such as electro chemical, bioelectrical, or reverse osmosis, would be useful to treat and recover nutrients from urine waste/wastewater. The laborious and costly affair of removing nutrients like N, P, and K from human urine needed a sustainable solution. These recovered nutrients can be reused as fertilizers in irrigation and, indirectly, in large-scale biodiesel production by being used in microalgae cultivation. However, the potential of reusing human urine waste is yet to be explored commercially. Additionally, artificial intelligence may be explored with sus tainable approaches for urine separation and recycling soon.
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    Current scenario and challenges in recycling of human urine generated at source in rail coaches as resource
    (2023-07) Dubey, K; Rajput, D; Baldia, A; Kumar, A
    The current scenario of human urine being directly discharged into the environment without recycling, despite being an economical source of fertilizer. Train coaches are the major source of large-scale urine waste generation. Adopting a cir cular economy creates significant synergies toward usages of water generated after nutrient recovery from urine. Some advanced decentralized treatment systems, such as electro chemical, bioelectrical, or reverse osmosis, would be useful to treat and recover nutrients from urine waste/wastewater. The laborious and costly affair of removing nutrients like N, P, and K from human urine needed a sustainable solution. These recovered nutrients can be reused as fertilizers in irrigation and, indirectly, in large-scale biodiesel production by being used in microalgae cultivation. However, the potential of reusing human urine waste is yet to be explored commercially. Additionally, artificial intelligence may be explored with sus tainable approaches for urine separation and recycling soon.
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    Insights into the mechanism of mycelium transformation of Streptomyces toxytricini into pellet
    (2023-08) Kumar, P; Khushboo; Rajput, D; Dubey, K
    Formation of the mycelial pellet in submerged cultivation of Streptomycetes is unwanted in industrial fermentation processes as it imposes mass transfer limitations, changes in the rheology of a medium, and affects the production of secondary metabolites. Though detailed information is not available about the factors involved in regulating mycelial morphology, it is studied that culture conditions and the genetic information of strain play a crucial role. Moreover,the proteomic study has revealed the involvement of low molecular weight proteins such as; DivIVA, FilP, ParA, Scy, and SsgA proteins in apical growth and branching of hyphae, which results in the establishment of the mycelial network. The present study proposes the mechanism of pellet formation of Streptomyces toxytricini (NRRL B-5426) with the help of microscopic and proteomic analysis. The microscopic analysis revealed that growing hyphae contain a bud like structure behind the apical tip, which follows a certain organized path of growth and branching, which was further converted into the pellet when shake flask to the shake flask inoculation was performed. Proteomic analysis revealed the production of low molecular weight proteins ranging between 20 and 95 kDa, which are involved in apical growth and hyphae branching and can possibly participate in the regulation of pellet morphology.