Insights into the mechanism of mycelium transformation of Streptomyces toxytricini into pellet
Loading...
Date
2023-08
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Formation of the mycelial pellet in submerged cultivation of Streptomycetes is unwanted in industrial fermentation processes as it
imposes mass transfer limitations, changes in the rheology of a medium, and affects the production of secondary metabolites. Though
detailed information is not available about the factors involved in regulating mycelial morphology, it is studied that culture conditions
and the genetic information of strain play a crucial role. Moreover,the proteomic study has revealed the involvement of low molecular
weight proteins such as; DivIVA, FilP, ParA, Scy, and SsgA proteins in apical growth and branching of hyphae, which results in the
establishment of the mycelial network. The present study proposes the mechanism of pellet formation of Streptomyces toxytricini (NRRL
B-5426) with the help of microscopic and proteomic analysis. The microscopic analysis revealed that growing hyphae contain a bud like structure behind the apical tip, which follows a certain organized path of growth and branching, which was further converted
into the pellet when shake flask to the shake flask inoculation was performed. Proteomic analysis revealed the production of low
molecular weight proteins ranging between 20 and 95 kDa, which are involved in apical growth and hyphae branching and can possibly
participate in the regulation of pellet morphology.