Browsing by Author "Kumari, S"

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    Biocompatible Drug Delivery System Based on a MOF Platform for a Sustained and Controlled Release of the Poorly Soluble Drug Norfloxacin
    (2023-07) Yadav, P; Kumari, S; Yadav, A; Bhardwaj, P; Maruthi, M
    Norfloxacin (NFX), an important antibacterial fluoroquinolone, is a class IVdrugaccording to thebiopharma ceutics classification system(BCS) and has low solubility and permeabilityissues.Suchpoorphysicochemicalpropertiesofdrug molecules leadtopoordeliveryandareof seriousconcerntothe pharmaceutical industryforclinicaldevelopment.Wepresenthere aconceptuallynewapproachtodeliverNFX,byloadingthedrug moleculeontheporousplatformofabiocompatiblemetal−organic framework(MOF),MIL-100(Fe).Theloadingof thedrugonthe MOFleadingtoNFX@MIL-100(Fe)wascharacterizedbyFourier transforminfrared(FTIR),UV−visiblespectroscopy, thermogravi metric analyses (TGA), and nitrogen adsorption studies. Controlledexperiments resulted in thehigh loadingof thedrug molecule(∼20wt%)alongwiththedesiredsustainedrelease.Wecouldfurthercontrol thereleaseofnorfloxacinbycoatingdrug loadedMIL-100(Fe) with PEG, PEG{NFX@MIL-100(Fe)}. Both drug delivery systems (DDSs), NFX@MIL-100(Fe) and PEG{NFX@MIL-100(Fe)},weretestedfortheirbiocompatibilitythroughtoxicitystudies.TheDDSsarebiocompatibleandshow insignificant cytotoxicity, as revealed by cell viability studies through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.
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    Biocompatible Drug Delivery System Based on a MOF Platform for a Sustained and Controlled Release of the Poorly Soluble Drug Norfloxacin
    (2023-07) Yadav, P; Kumari, S; Yadav, A; Bhardwaj, P
    ABSTRACT: Norfloxacin (NFX), an important antibacterial fluoroquinolone, is a class IV drug according to the biopharma ceutics classification system (BCS) and has low solubility and permeability issues. Such poor physicochemical properties of drug molecules lead to poor delivery and are of serious concern to the pharmaceutical industry for clinical development. We present here a conceptually new approach to deliver NFX, by loading the drug molecule on the porous platform of a biocompatible metal−organic framework (MOF), MIL-100(Fe). The loading of the drug on the MOF leading to NFX@MIL-100(Fe) was characterized by Fourier transform infrared (FTIR), UV−visible spectroscopy, thermogravi metric analyses (TGA), and nitrogen adsorption studies. Controlled experiments resulted in the high loading of the drug molecule (∼20 wt %) along with the desired sustained release. We could further control the release of norfloxacin by coating drug loaded MIL-100(Fe) with PEG, PEG{NFX@MIL-100(Fe)}. Both drug delivery systems (DDSs), NFX@MIL-100(Fe) and PEG{NFX@MIL-100(Fe)}, were tested for their biocompatibility through toxicity studies. The DDSs are biocompatible and show insignificant cytotoxicity, as revealed by cell viability studies through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
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    Integrated analysis of transcriptomic and small RNA sequencing data provides miRNA candidates for engineering agronomically important seed traits in Brassica juncea
    (2023-11) Jain, R; Dhaka, N; Yadav, P; Sharma, MK; Danish, MD; Sharma, S; Kumari, S
    Brassica juncea L. is an important oilseed crop that yields edible oil and biofuel. Improving B. juncea seed traits is a primary breeding target, but these traits are genetically complex. MicroRNAs (miRNAs) regulate seed devel opment by modulating gene expression at the post-transcriptional or translational level and are excellent can didates for improving seed traits. However, the roles of miRNAs in B. juncea seed development are yet to be investigated. Here, we report small RNA profiling and miRNA identification from developing seeds of two contrasting varieties of B. juncea, Early Heera2 (EH2) and Pusa Jaikisan (PJK). We identified 326 miRNAs, including 127 known and 199 novel miRNAs, of which 103 exhibited inter-varietal differential expression. Integrating miRNAome and our previous transcriptome data identified 13,683 putative miRNA-target modules. Segregation of differentially expressed miRNAs into different groups based on variety-wise upregulation, fol lowed by comprehensive functional analysis of targets using pathway mapping, gene ontology, transcription factor mapping, and candidate gene analysis, revealed at least 11, 6, and 7 miRNAs as robust candidates for the regulation of seed size, seed coat color, and oil content, respectively. Further, co-localization with previously reported quantitative trait loci (QTL) proffered 29 and 15 miRNAs overlapping with seed weight and oil content QTLs, respectively. Our study is the first comprehensive report of miRNAome expression dynamics from developing seeds and provides candidate miRNAs and target genes for engineering seed traits in B. juncea.