Transcriptome Analysis of Podoscypha petalodes Strain GGF6 Reveals the Diversity of Proteins Involved in Lignocellulose Degradation and Ligninolytic Function
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Date
2022-08
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Abstract
The present study reports transcriptomic profil
ing of a Basidiomycota fungus, Podoscypha petalodes strain
GGF6 belonging to the family Podoscyphaceae, isolated
from the North-Western Himalayan ranges in Himachal
Pradesh, India. Podoscypha petalodes strain GGF6 pos
sesses significant biotechnological potential as it has been
reported for endocellulase, laccase, and other lignocellulo
lytic enzymes under submerged fermentation conditions.
The present study attempts to enhance our knowledge of its
lignocellulolytic potential as no previous omics-based analy
sis is available for this white-rot fungus. The transcriptomic
analysis of P. petalodes GGF6 reveals the presence of 280
CAZy proteins. Furthermore, bioprospecting transcriptome signatures in the fungi revealed a diverse array of proteins
associated with cellulose, hemicellulose, pectin, and lignin
degradation. Interestingly, two copper-dependent lytic poly
saccharide monooxygenases (AA14) and one pyrroloquin
olinequinone-dependent oxidoreductase (AA12) were also
identified, which are known to help in the lignocellulosic
plant biomass degradation. Overall, this transcriptome pro
f
iling-based study provides deeper molecular-level insights
into this Basidiomycota fungi, P. petalodes, for its potential
application in diverse biotechnological applications, not only
in the biofuel industry but also in the environmental biodeg
radation of recalcitrant molecules.