Isolation, molecular identification and amino acid profiling of single-cell-protein-producing phototrophic bacteria isolated from oil-contaminated soil samples
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Date
2022
Journal Title
Journal ISSN
Volume Title
Publisher
Molecules
Abstract
the current study, soil samples were gathered from different places where petrol and
diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions
using the paraffin wax-overlay pour plate method with Biebl and Pfennig’s medium. The three isolated
strains were named Rhodopseudomonas palustris SMR 001 (Mallapur), Rhodopseudomonas palustris
NR MPPR (Nacahram) and Rhodopseudomonas faecalis N Raju MPPR (Karolbagh). The morphologies
of the bacteria were examined with a scanning electron microscope (SEM). The phylogenetic relationship
between R. palustris strains was examined by means of 16S rRNA gene sequence analysis using
NCBI-BLAST search and a phylogenetic tree. The sequenced data for R. palustris were deposited
with the National Centre for Biotechnology Research (NCBI). The total amino acids produced by
the isolated bacteria were determined by HPLC. A total of 14 amino acids and their derivatives
were produced by the R. palustris SMR 001 strain. Among these, carnosine was found in the highest
concentration (8553.2 ng/mL), followed by isoleucine (1818.044 ng/mL) and anserine (109.5 ng/mL),
while R. palustris NR MPPR was found to produce 12 amino acids. Thirteen amino acids and their
derivatives were found to be produced from R. faecalis N Raju MPPR, for which the concentration of
carnosine (21,601.056 ng/mL) was found to be the highest, followed by isoleucine (2032.6 ng/mL)
and anserine (227.4 ng/mL). These microbes can be explored for the scaling up of the process, along
with biohydrogen and single cell protein production.
Description
Keywords
phototrophic bacteria; molecular characterization; 16S rRNA gene sequence; amino acid profiling; SEM; HPLC