In silico mining and functional analysis of AP2/ERF gene in With ania somnifera

Simple item page
dc.contributor.authorTripathi, S
dc.contributor.authorSrivastava, Y
dc.contributor.authorSangwan, R
dc.contributor.authorSangwan, N
dc.date.accessioned2024-04-15T10:00:12Z
dc.date.available2024-04-15T10:00:12Z
dc.date.issued2020
dc.description.abstractWithania somnifera owing to its strong and remarkable stress tolerance property is a reliable candidate for the determination of genes involved in mechanism of adaption/tolerance of various stress conditions. 187 AP2/ERF gene related transcripts (GRTs) were identifed during comprehensive search in W. somnifera transcriptome repertoire. Major hits in homology search were observed from the model plant Arabidopsis and members of Solanaceae family. Cloning, expression analysis of the gene and genetic transient transformation with the gene (WsAP2) were performed to predict its functional role in planta. Enhanced expression of some of the pathway genes for terpenoid biosynthesis was observed in transformed tissues in comparison to the control tissues. It is speculated that WsAP2 gene crucially regulates the expression of GGPPS gene in addition to the regulation of other important genes of terpenoid pathway via induction of expression of other genes such as HMGR, CAS, DXS and DXR. To the best of our knowledge, this is the frst report representing detailed study of AP2/ERF gene family in W. somnifera. It is also suggested from the study that gene might have role in eliciting responses to combat stress and attribute the strong stress tolerant property associated with the plant.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1393
dc.language.isoenen_US
dc.titleIn silico mining and functional analysis of AP2/ERF gene in With ania somniferaen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
In_silico_mining_and_functional_analysis_of_AP2ERF[1].pdf
Size:
5.11 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
School of Interdisciplinary & Applied Sciences