Ultrasound-assisted extraction and RP-HPLC quantification of β-caryophyllene in plant essential oils: Separation efficiency and insecticidal activity

Simple item page
dc.contributor.authorKumar, Antresh
dc.date.accessioned2026-03-24T07:22:23Z
dc.date.available2026-03-24T07:22:23Z
dc.date.issued2025
dc.description.abstractThis study details the development of a method for separating essential oils using ultrasound-assisted extraction (UAE) and quantifying β-caryophyllene in 14 plant samples via reverse phase-high performance liquid chro matography (RP-HPLC). Extraction efficiencies of methods like hydrodistillation, Soxhlet extraction, and ultra sonic extraction were compared, with n-hexane, methanol, and ethyl acetate as solvents. Notably, UAE with n hexane showed the highest β-caryophyllene yield in HPLC analysis. Separation was achieved within 20 min using isocratic elution on a C-18 reverse-phase column, with a mobile phase of acetonitrile and water (70:30) at room temperature and detection at 210 nm via diode array. The method showed excellent linearity (0.1–5 µg/mL), with LOD and LOQ values of 0.02 and 0.07 µg/mL, respectively, achieving high accuracy (recovery rate of 102.1%) and precision (RSD 0.45%). Additionally, the insecticidal potential of β-caryophyllene, isolated from Hyptis suaveolens essential oil, was tested against two storage pests, Sitophilus granarius and Corcyra cephalonica larvae, demonstrating significant insecticidal activity. This suggests that β-caryophyllene could serve as an effective natural insecticide.
dc.identifier.urihttp://cuh.ndl.gov.in/handle/123456789/1832
dc.language.isoen
dc.titleUltrasound-assisted extraction and RP-HPLC quantification of β-caryophyllene in plant essential oils: Separation efficiency and insecticidal activity
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Ultrasound-assisted extraction and RP-HPLC quantification of β-caryophyllene in plant essential oils Separation efficiency and insecticidal activity.pdf
Size:
2.2 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
School of Interdisciplinary & Applied Sciences