Identification and fine‑mapping of novel major locus for yellow mosaic disease resistance in Glycine soja

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dc.contributor.authorSonah, Humaira
dc.date.accessioned2026-04-01T05:53:00Z
dc.date.available2026-04-01T05:53:00Z
dc.date.issued2026
dc.description.abstractKey message This is the first report on the identification and fine-mapping of yellow mosaic disease resistance locus, qMYMIV14.1, on chromosome 14 using integrative genomic approaches in interspecific soybean populations. Abstract Yellow mosaic disease (YMD) is a major viral threat to soybean production in Asian and Southeast Asian countries. Field screening of the disease was performed at Ludhiana; a YMD hot spot and its causative agent, mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) was detected and validated as the causal agent through PCR and sequence analysis. Genetic assessment was conducted on 1784 F2 plants derived from a cross between the susceptible cultivated soybean (Glycine max cv. ‘NRC 94’) and a resistant wild accession (Glycine soja accession ‘PI 393551’). The segregation ratio indicated that YMD resistance is controlled by four dominant genes, three of which confer resistance, while one inhibitory gene suppresses this resistance. Test of allelism performed on direct and reciprocal crosses [SL 958//JS 335/PI 393551 (BC5F6)] across F1, F2, and F3 generations revealed that genes in cultivated and wild soybean were non-allelic with no maternal effect. Bulked segregant analysis (BSA) initially identified eight markers (Satt157, BARCSOYSSR_14_0441, BARCSOYSSR_14_0445, BARCSOYSSR_14_0448, BARCSOYSSR_14_0455, BARCSOYSSR_14_1416, BARC SOYSSR_14_1417 & BARCSOYSSR_14_1516) linked to resistance. Traditional QTL mapping revealed three novel QTLs on chromosome 14. Combined results from QTL-seq, a genome-wide association study, and QTL mapping consistently identified a major and stable locus, qMYMIV14.1, spanning the 46.55–48.70 Mb region on the long arm of chromosome 14. This is the first QTL detected from an interspecific cross which explained for 27.81–68.01% of phenotypic variance. Two candidate genes, Glyma.14G173300 and Glyma.14G173600, encoding leucine-rich repeat proteins, were identified within this locus. These findings provide valuable genomic resources for marker-assisted selection and breeding of soybean cultivars with durable YMD resistance.
dc.identifier.urihttp://cuh.ndl.gov.in/handle/123456789/1862
dc.language.isoen
dc.titleIdentification and fine‑mapping of novel major locus for yellow mosaic disease resistance in Glycine soja
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