Enhanced production of lipstatin from mutant of Streptomyces toxytricini and fed‑batch strategies under submerged fermentation

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dc.contributor.authorKhushboo
dc.contributor.authorPinki
dc.contributor.authorKumar, Punit
dc.contributor.authorDubey, Kashyap.Kumar
dc.contributor.authorLuthra, Umesh
dc.date.accessioned2023-05-01T18:13:54Z
dc.date.available2023-05-01T18:13:54Z
dc.date.issued2020
dc.description.abstractStreptomyces toxytricini produces bioactive metabolite recognized as lipstatin and its intermediate orlistat. The main focus of this study is to enhance lipstatin production by strain improvement and precursor feeding. In this study, strain improvement to enhance the production of lipstatin was carried out by different doses (50, 100, 150, 200, and 250 Gy) of gamma radiation and precursors (Linoleic acid, Oleic acid, and l-Leucine). Screening showed that the highest yield of lipstatin (4.58 mg/g) was produced by mutant designated as SRN 7. The production of lipstatin (5.011 mg/g) increased significantly when the medium was supplemented with ratio 1:1.5 (linoleic acid + oleic acid). The addition of 1.5% l-Leucine leads to further increment in the production of lipstatin (5.765 mg/g). The addition of 10% soy flour in the culture medium resulted in the maximum production of lipstatin to 5.886 mg/g.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1061
dc.language.isoenen_US
dc.publisher3 Biotechen_US
dc.subjectAntiobesity drug · Fatty acid precursors · Gamma radiation · Precursors · Lipstatinen_US
dc.titleEnhanced production of lipstatin from mutant of Streptomyces toxytricini and fed‑batch strategies under submerged fermentationen_US
dc.typeArticleen_US
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