Enhanced production of lipstatin from mutant of Streptomyces toxytricini and fed‑batch strategies under submerged fermentation
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
3 Biotech
Abstract
Streptomyces toxytricini produces bioactive metabolite recognized as lipstatin and its intermediate orlistat. The main focus
of this study is to enhance lipstatin production by strain improvement and precursor feeding. In this study, strain improvement
to enhance the production of lipstatin was carried out by different doses (50, 100, 150, 200, and 250 Gy) of gamma
radiation and precursors (Linoleic acid, Oleic acid, and l-Leucine). Screening showed that the highest yield of lipstatin
(4.58 mg/g) was produced by mutant designated as SRN 7. The production of lipstatin (5.011 mg/g) increased significantly
when the medium was supplemented with ratio 1:1.5 (linoleic acid + oleic acid). The addition of 1.5% l-Leucine leads to
further increment in the production of lipstatin (5.765 mg/g). The addition of 10% soy flour in the culture medium resulted
in the maximum production of lipstatin to 5.886 mg/g.
Description
Keywords
Antiobesity drug · Fatty acid precursors · Gamma radiation · Precursors · Lipstatin