Current perspectives on chitinolytic enzymesand their agro-industrial applications
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Biology
Abstract
Chitinases are a large and diversified category of enzymes that break down chitin, the
world’s second most prevalent polymer after cellulose. GH18 is the most studied family of chitinases,
even though chitinolytic enzymes come from a variety of glycosyl hydrolase (GH) families. Most of
the distinct GH families, as well as the unique structural and catalytic features of various chitinolytic
enzymes, have been thoroughly explored to demonstrate their use in the development of tailor-made
chitinases by protein engineering. Although chitin-degrading enzymes may be found in plants and
other organisms, such as arthropods, mollusks, protozoans, and nematodes, microbial chitinases are
a promising and sustainable option for industrial production. Despite this, the inducible nature, low
titer, high production expenses, and susceptibility to severe environments are barriers to upscaling
microbial chitinase production. The goal of this study is to address all of the elements that influence
microbial fermentation for chitinase production, as well as the purifying procedures for attaining
high-quality yield and purity.
Description
Keywords
chitinolytic enzymes; microbial chitinases; chitooligosaccharides; fermentation; biocontrol