Implication of mutagenesis and precursor supplementation towards the enhancement of lipstatin (an antiobesity agent) biosynthesis through submerged fermentation using Streptomyces toxytricini

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dc.contributor.authorKumar, Punit
dc.contributor.authorDubey, Kashyap.Kumar
dc.date.accessioned2023-05-01T18:47:04Z
dc.date.available2023-05-01T18:47:04Z
dc.date.issued2018
dc.description.abstractIn the present study, lipstatin production was studied from different mutants of Streptomyces toxytricini which were developed using ultraviolet radiation (exposure time 30 s, 1, 2, 5 and 10 min), ethyl methane sulfonte, methyl methane sulfonate (MMS) and N-methyl-N′-intro-N-nitrosoguanidine (NTG) treatments (50, 100, 200, 500, 1000 μM, respectively). Highest yielding mutants were provided precursor supplementation of citric acid, thiamine and biotin (each 1 g/L) at idiophase for further enhancement in the production of lipstatin. Screened mutants produced biomass in the range of 5.8–7.16 g/L which were lesser than control. Screened mutants also exhibited pellet morphology in submerged culture. Out of these mutants, NTG8 mutant produced highest amount of lipstatin (1383.25 mg/L) with 9.606 mg/L/h productivity. Precursor supplementation to this mutant further increased the production to 2387.81 mg/L. Mutant was validated in 5 L bioreactor and lipstatin production was enhanced to 2519.34 mg/L.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1065
dc.language.isoenen_US
dc.publisher3 Biotechen_US
dc.subjectObesity · Lipstatin · Orlistat · Streptomyces toxytricini · Mutagenesis · Precursor supplementationen_US
dc.titleImplication of mutagenesis and precursor supplementation towards the enhancement of lipstatin (an antiobesity agent) biosynthesis through submerged fermentation using Streptomyces toxytricinien_US
dc.typeArticleen_US
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