Chikungunya virus non-structural protein nsP3 interacts with Aedes aegypti DEAD-box helicase RM62F
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Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Virus Disease
Abstract
The non-structural proteins (nsPs) of the
chikungunya virus (CHIKV) form the virus’s replication
complex. They are known to participate in several functions
that allow efficient replication of the virus in diverse
host systems. One such function is evading the host defense
system such as RNA interference (RNAi). Two nsPs of
CHIKV, namely, nsP2 and nsP3, were found to suppress
the host/vector RNAi machinery and exhibit RNAi suppressor
activity. The present study was undertaken to
identify interacting partners of CHIKV-nsP3 in Aedes
aegypti. We performed pull-down assays with the mass
spectrometry approach and showed the interaction of
CHIKV-nsP3 with several Aedes proteins. Further co-immunoprecipitation
assays revealed that CHIKV-nsP3
interacts with RM62F, a DEAD-box containing RNA
known to play roles in multiple gene regulatory processes
such as alternative splicing, RNA release, and also is a
component of Ago2-RISC complex.
Description
Keywords
Chikungunya virus Aedes aegypti nsP3 RNA interference Arboviruses