Evaluation of bioactive secondary metabolites from endophytic fungus Pe stalotiopsis neglect a BAB-5510 isolated from leaves of Cupressus torulosa D.Don
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Date
2016
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3 Biotech
Abstract
Six endophytic fungi were isolated from Cupressus
torulosa D.Don and identified phenotypically and
genotypically. The fungal cultures were further grown and
the culture was extracted by two organic solvents methanol
and ethyl acetate. The screening was carried out using the
agar well diffusion method against human pathogen such
as Escherichia coli, Salmonella typhimurium, Bacillus
subtilis and Staphylococcus aureus. Isolated strain of
Pestalotiopsis sp. was showing prominent antibacterial
activity. The crude methanol and ethyl acetate extract of
Pestalotiopsis sp. showed MIC of 6.25 mg/mL for S.
typhimurium and S. aureus which showed its efficacy as a
potent antimicrobial. The phytochemical screening
revealed the existence of a diverse group of secondary
metabolites in the crude extracts of the endophytic fungi
that resembled those in the host plant extracts. On the basis
of phenotypic characteristics and rDNA sequencing of the
ITS region of the endophyte was identified as P. neglecta
which turned out to be a promising source of bioactive
compounds. There is little known about endophytes from
C. torulosa D.Don. In this paper we studied in detail the
identification of isolated endophytic fungi P. neglecta from
C. torulosa D.Don and characterization of its active
metabolite compounds. The partially purified second fraction
(PPF) extracted from the fungal culture supernatant
was subjected to gas chromatography followed by mass
spectrometry which revealed the presence of many
phytochemicals. These results indicate that endophytic
fungi P. neglecta isolated from medicinal plants could be a
potential source for bioactive compounds and may find
potential use in pharmaceutical industry
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Keywords
Pestalotiopsis neglecta Antibacterial activity Cytotoxic assay GC-MS analysis Bioactive metabolites