Browsing by Author "Kumar, Ramesh"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Chikungunya virus non-structural protein nsP3 interacts with Aedes aegypti DEAD-box helicase RM62F
    (Virus Disease, 2021) Kumar, Ramesh; Kaur, Inderjeet; Chattopadhyay, Soma; Nayak, Debasis; Mathur, Kalika
    The non-structural proteins (nsPs) of the chikungunya virus (CHIKV) form the virus’s replication complex. They are known to participate in several functions that allow efficient replication of the virus in diverse host systems. One such function is evading the host defense system such as RNA interference (RNAi). Two nsPs of CHIKV, namely, nsP2 and nsP3, were found to suppress the host/vector RNAi machinery and exhibit RNAi suppressor activity. The present study was undertaken to identify interacting partners of CHIKV-nsP3 in Aedes aegypti. We performed pull-down assays with the mass spectrometry approach and showed the interaction of CHIKV-nsP3 with several Aedes proteins. Further co-immunoprecipitation assays revealed that CHIKV-nsP3 interacts with RM62F, a DEAD-box containing RNA known to play roles in multiple gene regulatory processes such as alternative splicing, RNA release, and also is a component of Ago2-RISC complex.
  • Thumbnail Image
    Item
    Proficient exclusion of pesticide using humic acid-modified magnetite nanoparticles from aqueous solution
    (Water Science and Technology, 2022) Jangra, Arti; Kumar, Jai; Singh, Devender; Kumar, Harish; Kumar, Parvin; Kumar, Suresh; Kumar, Ramesh
    Extensive dispersal of the pesticides to shield the different types of vegetation from pests has increased the production but at the same it has resulted into increase in environmental pollution. Consequently, it is necessary to eliminate these undesired pollutants from the environment. The current investigation offers the synthesis of humic acid-coated magnetite nanoparticles towards effective removal of the most common insecticide, imidacloprid from the aqueous solution using batch adsorption method. These synthesized nanoparticles were characterized with the help of several analytical and spectroscopic techniques. To acquire the maximum conceivable adsorption, effects of different influencing parameters like pH of the solution, time of contact, concentration of pesticide solution, amount of adsorbent and temperature were also examined. Moreover, the kinetic studies were found to be in good agreement with pseudo-second-order kinetic model supporting the occurrence of chemisorption phenomenon. Additionally, isotherm modeling proved that the adsorption process was in accordance with Langmuir model of isotherm. Thermodynamic parameters depicted the endothermic and spontaneous behavior of the adsorption process. Desorption studies were also carried out to examine the reusability of these nano-adsorbent. These verdicts confirmed that the surface modified magnetite nanoparticles may be treated as proficient material for exclusion of imidacloprid from the aqueous solution.