Browsing by Author "Saini, M"
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Item Bioacetoin Production by Bacillus subtilis subsp. subtilis Using Enzymatic Hydrolysate of Lignocellulosic Biomass(2023-07) Saini, M; Rapoport, A; Tiwari, S; Singh, DAbstract: Acetoin is an important bio-product useful in the chemical, food and pharmaceutical industries. Microbial fermentation is the major process for the production of bioacetoin, as the petroleum resources used in chemical methods are depleting day by day. Bioacetoin production using wild microorganisms is an easy, eco-friendly and economical method for the production of bioacetoin. In the present study, culture conditions and nutritional requirements were optimized for bioacetoin production by a wild and non-pathogenic strain of B. subtilis subsp. subtilis JJBS250. The bacterial culture produced maximum bioacetoin (259 mg L−1 ) using peptone (3%) and sucrose (2%) at 30 ◦C, 150 rpm and pH 7.0 after 24 h. Further supplementation of combinatorial nitrogen sources, i.e., peptone (1%) and urea (0.5%), resulted in enhanced titre of bioacetoin (1017 mg L−1 ) by the bacterial culture. An approximately 46.22–fold improvement in bioacetoin production was achieved after the optimization process. The analysis of samples using thin layer chromatography confirmed the presence of bioacetoin in the culture filtrate. The enzymatic hydrolysate was obtained by saccharification of pretreated rice straw and sugarcane bagasse using cellulase from Myceliophthora thermophila. Fermentation of the enzymatic hydrolysate (3%) of pretreated rice straw and sugarcane bagasse by the bacterial culture resulted in 210 and 473.17 mgL−1 bioacetoin, respectively. Enzy matic hydrolysates supplemented with peptone as a nitrogen source showed a two to four-fold improvement in the production of bioacetoin. Results have demonstrated the utility of wild type B. subtilis subsp. subtilis JJBS250 as a potential source for economical bioacetoin production by making use of renewable and cost-effective lignocellulosic substrate. Therefore, this study will help in the sustainable management of agricultural waste for the industrial production of bioacetoin, and in combating environmental pollution.Item Improvement of power quality of a 200 kW grid-connected PV system(2023-09) Saini, M; Khan, S; Sharma, AIn order to enhance electricity quality, a grid-connected photovoltaic (PV) system simulation is presented in this study. A 200 kW photovoltaic system is integrated to a utility grid and loads. A 25 kV 3-level insulated gate bipolar transistor (IGBT) bridge converter is used. Through this system integration of a renewable energy source with a non-renewable source is achieved. This system also wins over the intermittent nature of the renewable source (solar energy) and non-reliability of conventional sources (utility grid). Power and signal quality for various signals such as ripple factor and total harmonic distortion has improved. The suggested system is investigated using MATLAB/Simulink environment. Total harmonic distortion (THD) in voltage and current, ripple factor correction is also incorporate in the MATLAB model.Item Improvement of power quality of a 200 kW grid-connected PV system(2023-04) Saini, M; Khan, S; Sharma, AK; Chaudhary, SK; Srivastava, K; Srivastava, P; Saini, SIn order to enhance electricity quality, a grid-connected photovoltaic (PV) system simulation is presented in this study. A 200 kW photovoltaic system is integrated to a utility grid and loads. A 25 kV 3-level insulated gate bipolar transistor (IGBT) bridge converter is used. Through this system integration of a renewable energy source with a non-renewable source is achieved. This system also wins over the intermittent nature of the renewable source (solar energy) and non-reliability of conventional sources (utility grid). Power and signal quality for various signals such as ripple factor and total harmonic distortion has improved. The suggested system is investigated using MATLAB/Simulink environment. Total harmonic distortion (THD) in voltage and current, ripple factor correction is also incorporate in the MATLAB model.Item Stochastic modeling and parameter estimation of turbogenerator unit of a thermal power plant under classical and Bayesian inferential framework(2023-10) Kumar, A; Chaudhary, R; Kumar, K; Saini, M; Saini, DThe work reported in present study deals with the development of a novel stochastic model and estimation of parameters to assess reliability characteristics for a turbogenerator unit of thermal power plant under classical and Bayesian frameworks. Turbogenerator unit consists of five components namely turbine lubrication, turbine governing, generator oil system, gen erator gas system and generator excitation system. The concepts of cold standby redun dancy and Weibull distributed random variables are used in development of stochastic model. The shape parameter for all the random variables is same while scale parameter is different. Regenerative point technique and semi-Markov approach are used for evaluation of reliability characteristics. Sufficient repair facility always remains available in plant as well as repair done by the repairman is considered perfect. As the life testing experiments are time consuming, so to highlight the importance of proposed model Monte Carlo simulation study is carried out. A comparative analysis is done between true, classical and Bayesian results of MTSF, availability and profit function.