Browsing by Author "Gunawan, W"

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    Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha
    (2022-08) Permatasari, H; Nurkolis, F; Gunawan, W; Yusuf, V; Yusuf, M; Kusuma, R; Sabrina, N; Muharram, F; Taslim, N; Mayulu, N; Batubara, S; Samtiya, M
    Clitoria ternatea, with an alternative name, Butterfly pea, is increasingly being explored for medical purposes and the development of a wide range of processed products. This study aimed to incorporate Butterfly pea into an innovative probiotic drink through a symbiotic culture of bacteria and yeast (SCOBY) fermentation and to evaluate the biological activity. The benefits of the drink, referred to as butterfly pea flower kombucha (KBPF) was determined in vitro and in metabolically disorder mice that receive a diet rich in cholesterol and fat (CFED). Forty white male were categorized into four groups, i.e., A =Control/Normal Diet; B =CFED alone; C =CFED + KBPF 65 mg/kg BW (Body Weight); D =CFED +KBPF 130 mg/kg BW, and then sacrificed after 6 weeks of intervention. Seventy-nine secondary metabolite compounds were successfully identified in KBPF using LC- HRMS. In vitro studies showed the potential activity of KBPF in inhibiting not only ABTS, but also lipid (lipase) and carbohydrate (α-amylase, α-glucosidase) hydrolyzing enzymes to levels similar to acarbose control at 50–250 μg/mL. In the in vivo study, the administration of KBPF (130 mg/kg BW) significantly alleviated metabolic disorders caused by high-fat diet. Specifically, lipid profile (HDL, LDL, TC, TG), blood glucose, markers of oxidative stress (SOD liver), metabolic enzymes (lipase, amylase), and markers of inflammation (PGC-1α, TNF- α, and IL-10) were in most cases restored to normal values. Additionally, the gut microbiota community analysis showed that KBPF has a positive effect (p =0.01) on both the Bacteroidetes phylum and the Firmicutes phylum. The new KBPF drink is a promising therapeutic functional food for preventing metabolic diseases.
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    Rice field snail shell anticancer properties: An exploration opinion
    (2023-01) Samtiya, M; Rompas, J; Laatung, S; Gunawan, W
    Mollusks, especially Gastropods – which include land, freshwater, and sea snails – are commonly used as traditional medicine and cost-effective food resource (1). Snail meat has beneficial nutritional values as it is high in protein and low in fat (2). Hence markets around Asia, such as Indonesia, China, Taiwan, Japan, and Hongkong, frequently process snail meat into food (3). A type of freshwater snail, Pila ampullacea, is a native mollusk easily found in Southeast Asian rice fields and lakes. Like its fellow gastropods, it contains high nutritional values (100 mg meat: ± 209 kcal calories, ± 18 g protein, 12 mg zinc, 102 mg iron, and 812 mg calcium) (4). Although conventionally consumed as food, Pila ampullacea is also recognized as a crop pest (5). The only component of snails that has been incorporated into food is snail meat (6, 7). As their consumption rises, snail shells are less explored functionally and become animal feed material, accessories, and waste products with low economic value (1). Calcium carbonate constitutes 87-96% of the total weight of freshwater snail shells (8). High dietary calcium intake is clinically protective against multiple chronic diseases, including lowering the risks of developing cancer (9, 10). Calcium carbonate is also the primary material used to synthesize hydroxyapatite, a biocompatible material with high binding activity to proteins and genetic materials.